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个人简介 宗鑫，博士，浙江大学特聘研究员，博士生导师。主持国家自然科学基金面上项目、青年基金项目、“十四五”重点研发子课题、浙江省自然科学基金探索项目等10余项。聚焦于断奶仔猪肠道健康，围绕“免疫-肠道微生态-表观遗传”之间的关系，开展断奶应激、病原微生物感染等造成仔猪肠道免疫功能紊乱和营养物质吸收不良的分子生物学机制及其营养调控研究。近年来，在Nucleic Acids Research、Journal of Advanced Research、SCIENCE CHINA Life Sciences、Journal of Immunology、Animal Nutrition、PNAS等权威期刊发表论文30余篇；授权发明专利3项，其中美国专利1项。担任Frontiers in Bioscience-Landmark等杂志编委；Journal of Animal Science、Animal Nutrition、Frontiers in Immunology、动物营养学报等多个国内外权威期刊的审稿专家。教育背景2013.09-2019.09   农学博士     浙江大学（动物营养与饲料科学）  中国2016.09-2017.09  联合培养   康奈尔大学（Nutritional Science） 美国2009.09-2013.07   理学学士     华南农业大学（生物科学）         中国工作经历2019.09-2021.07    浙江大学动物科学学院  博士后2021.07-至今        浙江大学动物科学学院  研究员 教学与课程 1. 本科生课程：动物营养与免疫2. 本科生课程：动物性食品安全3. 研究生课程：现代农业创新与乡村振兴战略 研究方向       依托于绿色饲料与健康养殖国家工程研究中心、教育部动物分子营养学重点实验室和农业农村部（华东）动物营养与饲料重点实验室；主要围绕仔猪肠道免疫和养分高效利用，聚焦表观遗传和营养调控开展研究；致力于阐明表观遗传-肠道免疫-营养物质吸收的互作网络，保证无抗时代生猪的健康养殖。1、肠道免疫调控断奶仔猪养分高效利用的机制探究      通过比较商品猪与地方品种猪，构建断奶、细菌感染仔猪和小鼠模型，探究仔猪肠道免疫应答与脂肪酸吸收的关系，发现免疫应激能够显著降低猪肠道脂肪酸，特别是中长链脂肪酸的吸收，而且往往伴随着肠道菌群紊乱；通过基因缺失小鼠联合粪菌移植等实验解析了肠道菌群和肠道免疫互作调控肠道脂肪酸吸收的分子机制，筛选到了Romboutsia和Lactobacillus等调控肠道脂肪酸吸收的关键菌株和代谢产物。该研究阐明了肠道免疫调控猪肠道脂肪酸吸收的肠道微生物机制；相关成果发表在Journal of Immunoloy、Microbial Biotechnology等杂志。 2、猪肠道免疫的表观遗传学机制解析      通过建立猪的体外肠道上皮细胞极化模型，结合代谢组学和基因沉默技术，创新性地发现猪肠道炎症反应的激活伴随着RNA甲基化修饰水平的升高，降低RNA甲基化修饰水平能够显著降低炎症反应，并改善肠道脂肪酸吸收；解析内在机制发现，RNA甲基化是通过控制TRAF6 mRNA的出核，调控其蛋白的表达，激活NF-κB信号通路，调节炎症反应，进而影响脂肪酸吸收。针对RNA甲基化的各个效应蛋白进行筛选，发现RNA甲基化是通过YTHDF1参与调控肠道免疫应答；进一步利用翻译组、RNA甲基化组、蛋白组以及点突变等技术，发现YTHDF1是通过其P/Q/N结构域与DDX60相互作用识别TRAF6、TRAF3、NLRP3 mRNA上的m6A修饰，调控蛋白质翻译过程，启动免疫应答，抵御病原微生物的感染。该研究系统解析了肠道免疫反应的表观遗传学机制，筛选得到了YTHDF1、DDX60、TRAF6、NF-κB等一系列调控肠道免疫的关键基因，为营养调控仔猪免疫力提供了靶点；相关成果发表在Nucleic Acids Research、Science China-Life Sciences等杂志。3、改善仔猪养分利用和肠道免疫的营养素筛选及作用机制解析      依据上述解析的肠道免疫应答机制，通过构建猪肠道上皮细胞、类器官和小鼠的肠道损伤模型，挖掘得到多糖、抗菌肽、益生菌等能够显著改善猪肠道免疫的营养素，并解析了内在分子机制，形成了一套调控肠道免疫的营养策略，为保障无抗时代生猪的健康养殖提供了新的理论支撑；相关研究成果发表于Animal Nutrition、International Journal of Biological Macromolecules、Microbial Biotechnology等杂志，授权国内/国际专利3项。诚挚欢迎各位同学联系毕设及报考研究生！！！ 研究与成果 发表文章：(17) Xin Zong, Xiao Xiao, Bin Shen, Qin Jiang, Hong Wang, Zeqing Lu, Fengqin Wang, Mingliang Jin, Junxia Min, Fudi Wang*, Yizhen Wang*. The N6-methyladenosine RNA-binding protein YTHDF1 Modulates the Translation of TRAF6 to Mediate the Intestinal Immune Response. Nucleic Acids Research. 2021, gkab343.(16) Xin Zong, Xiao Xiao, Fu Jie, Yuanzhi Cheng, Mingliang Jin, Yulong Yin*, Yizhen Wang*. YTHDF1 promotes NLRP3 translation to induce intestinal epithelial cell inflammatory injury during endotoxic shock. SCIENCE CHINA Life Sciences. 2021, 1-4.(15) Jie Fu#, Tenghao Wang#, Xiao Xiao, Yuanzhi Cheng, Fengqin Wang ,Mingliang Jin, Xin Zong*. Clostridium Butyricum ZJU-F1 Benefits the Intestinal Barrier Function and Immune Response Associated with Its Modulation of Gut Microbiota in Weaned Piglets. Cells, 2021, 10, 527.(14) Tenghao Wang#, Jie Fu#, Xiao Xiao, Zeqing Lu, Fengqin Wang, Mingliang Jin, Yizhen Wang*, Xin Zong*. CBP22, a novel bacteriocin isolated from Clostridium butyricum ZJU-F1, protects against LPS-induced intestinal injury through maintaining the tight junction complex. Mediators of Inflammation, 2021, 10, 527.(13) Xiao Xiao, Yuanzhi Cheng, Jie Fu, Zeqing Lu, Fengqin Wang, Mingliang Jin, Xin Zong*, Yizhen Wang*. Gut Immunity and Microbiota Dysbiosis Are Associated with Altered Bile Acid Metabolism in LPS-Challenged Piglets. Oxidative Medicine and Cellular Longevity, 2021. 6634821.(12) Quanquan Ji, Xin Zong, Yuanhui Mao, Shu-Bing Qian*. A heat shock-responsive lncRNA Heat acts as a HSF1-directed transcriptional brake via m6A modification. PNAS, 2021, 25, 141-147.(11) Xin Zong, Xiao Xiao, Lixia Kai, Yuanzhi Cheng, Jie Fu, Wei Xu, Yizhen Wang, Ke Zhao*, Mingliang Jin*. Atractylodis macrocephalae polysaccharides protect against DSS-induced intestinal injury through a novel lncRNA ITSN1-OT1. International Journal of Biological Macromolecules. 2021, 167, 76-84.(10) Xin Zong#, Hong Wang#, Xiao Xiao, Yu Zhang, Yuhan Hu, Fengqin Wang, Yizhen Wang, Zeqing Lu*. Enterotoxigenic Escherichia coli infection promotes enteric defensin expression via FOXO6-METTL3-m6A-GPR161 signalling axis. RNA biology. 2021, 1-11.(9) Xin Zong, Yuanzhi Cheng, Xiao Xiao, Jie Fu, Fengqin Wang, Zeqing Lu, Yizhen Wang*, Mingliang Jin*. Protective effects of sulfated polysaccharide from Enterobacter cloacae Z0206 against DSS-induced intestinal injury via DNA methylation. International Journal of Biological Macromolecules. 2021, 31, 861-869.(8) Xin Zong, Jie Fu, Bocheng Xu, Yizhen Wang, Mingliang Jin*. Interplay between gut microbiota and antimicrobial peptides. Animal Nutrition. 2020, 6, 389-396.(7) Xin Zong#, Xiaoxuan Cao#, Hong Wang, Xiao Xiao, Yizhen Wang*, Zeqing Lu*. Cathelicidin-WA facilitated intestinal fatty acid absorption through enhancing PPAR-γ dependent barrier function. Frontiers in Immunology. 2019, 10, 1674.(6) Xin Zong, Jing Zhao, Hong Wang, Zeqing Lu, Fengqin Wang, Huahua Du, Yizhen Wang*. Mettl3 Deficiency Sustains Long-Chain Fatty Acid Absorption through Suppressing Traf6-Dependent Inflammation Response. Journal of Immunology. 2019, 202 (2) 567-578.(5) Xin Zong, Hong Wang, Jing Zhao, Zeqing Lu, Fengqin Wang, Yizhen Wang*. Porcine lactoferrin-derived peptide LFP-20 modulates immune homoeostasis to defend lipopolysaccharide-triggered intestinal inflammation in mice. British Journal of Nutrition. 2019, 121, 1255–1263.(4) Xin Zong#, TengHao Wang#, Zeqing Lu, Deguang Song, Jin Zhao, Yizhen Wang*. Effects of Clostridium butyricum or in combination with Bacillus licheniformis on the growth performance, blood indexes, and intestinal barrier function of weanling piglets. Livestock Science. 2019, 220, 137-142(3) Xin Zong, Wangyang Hu, Deguang Song, Zhi Li, Huahua Du, Zeqing Lu, Yizhen Wang*. Porcine lactoferrin-derived peptide LFP-20 protects intestinal barrier by maintaining tight junction complex and modulating inflammatory response. Biochemical Pharmacology. 2016, 104, 74-82.(2) Xin Zong, Deguang Song, Tenghao Wang, Feifei Han, Yizhen Wang*. LFP-20, a porcine lactoferrin peptide, ameliorates LPS-induced inflammation via the MyD88/NF-κB and MyD88/MAPK signaling pathways. Developmental & Comparative Immunology, 2015, 52, 123–131.(1) 宗鑫，胡汪洋，汪以真; 猪髓样分化因子MyD88特异性shRNA干扰载体的构建筛选及干扰效果评价. 中国生物工程杂志, 2015, 7,1-7.     其他参与文章：(7) Deguang Song, Xin Zong, Haiwen Zhang, Tenghao Wang, Hongbo Yi, Chao Luan, Yizhen Wang*. Antimicrobial peptide Cathelicidin-BF prevents intestinal barrier dysfunction in a mouse model of endotoxemia. International immunopharmacology, 2015, 25, 141-147.(6) Nana Chen, Hao Zhang, Xin Zong, Siyu Li, Jiaojiao Wang, Yizhen Wang, Mingliang Jin*. Polysaccharides from Auricularia auricula: Preparation, structural features and biological activities. Carbohydrate Polymers, 2020, 116750.(5) Yuhan Hu, Xiangyun Huang, Xin Zong, Zhen Bi, Yuanzhi Cheng, Xiao Xiao, Fengqin Wang, Yizhen Wang, Zeqing Lu*. Chicory fibre improves reproductive performance of pregnant rats involving in altering intestinal microbiota composition. Journal of applied microbiology, 2020, 6, 1693-1705.(4) Yu Zhang, Shan Chen, Xin Zong, Cheng Wang, Changyou Shi, Fengqin Wang, Yizhen Wang, Zeqing Lu*. Peptides derived from fermented soybean meal suppresses intestinal inflammation and enhances epithelial barrier function in piglets. Food and Agricultural Immunology, 2020, 31, 120-135.(3) Jianzhao Liu#, Yuanxiang Zhu#, Guan-Zheng Luo#, Xinxia Wang#, Yanan Yue, Xiaona Wang, Xin Zong, Kai Chen, Hang Yin, Ye Fu, Dali Han, Yizhen Wang*, Dahua Chen*, Chuan He*. Abundant DNA 6mA methylation during early embryogenesis of zebrafish and pig. Nature communications, 2016, 1, 1-7.(2) Feifei Han, Haiwen Zhang, Xi Xia, Haitao Xiong, Deguang Song, Xin Zong, Yizhen Wang*. Porcine β-defensin 2 attenuates inflammation and mucosal lesions in dextran sodium sulfate–induced colitis. Journal of Immunology, 2015,194, 1882-1893.(1) 王腾浩, 宗鑫, 宋德广, 汪以真; 产抑菌蛋白的丁酸梭菌的筛选和鉴定及体外益生功能研究, 中国畜牧杂志, 2015, 13, 75-81. 授权专利：(1) 宗鑫，汪以真，赵婧. METTL3基因敲除的细胞系及其构建方法与干扰载体. 中国. 201810733097.5, 发明专利, 已授权.(2) 汪以真，宗鑫，王腾浩；高产抗菌肽地衣芽孢杆菌及其应用. 中国. ZL201410653450.0, 发明专利, 已授权 (转化至江苏三仪生物工程有限公司).